Placental derived Mesenchymal Stem/ Stromal Cells (MSC)
Mesenchymal stem cells (MSCs) also known as mesenchymal stromal cells or medicinal signaling cells are multipotent stromal cells.
Human Umbilical Vein Endothelial Cells (HUVEC)
HUVEC are isolated from placentas collected after full-term, uncomplicated birth, from volunteer donors. The isolated cells are stored in vapor-state LN2 and available for further research applications.
Amnion
Amnion is excised from placental organ, washed, and stored frozen for further research applications. Amnion is not cut into small sheets, but available as a full membrane from consented donors.
FAQ
How are these products sourced?
All cellular products are sourced from volunteer donors who are qualified through an independent institutional review board (IRB) or through administration and completion of a Donor Risk Assessment Interview (DRAI).
How are these products isolated?
Mesenchymal Stromal/Stem Cells (MSC) are isolated, digested, washed, and expanded through validated standard operating procedures. Passages and doubling may be performed manually in successive flasks or through the use of a Quantum bioreactor.
How are the products stored?
MSC and HUVEC are vialed and stored in vapor state LN2.
Amnion is excised and frozen in -20C freezer.
How can the MSC and HUVEC be used?
Cells are vialed in 1×10^6 or 5×10^6 aliquots and can be used for further research applications. Cells should not be used in clinical applications at this time.
What characteristics do the MSC possess?
MSC are CD34 Negative, CD44 Positive, CD73 Positive, CD90 Positive, and HLA-DR Negative. Additionally, they are tested and found to be negative for mycoplasma and endotoxin.
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Placental derived Mesenchymal Stem/ Stromal Cells (MSC)
Storage
20C or in vapor state LN2 until shipped on dry ice
Sterility
Cells are tested and found to be negative for mycoplasma and endotoxin.
Infectious Diseases
Donor tested and found to be negative for HIV
1, HIV 2, Hepatitis B, Hepatitis C, HTLV 1, WNV,
Zika, and Syphilis Testing is performed in an FDA approved and regulated laboratory.
Methods
These mesenchymal stromal cells (MSC) are isolated from human placenta, digested with trypsin then cultured an d expanded in culture media. The MSC are passed to successively larger flasks after achieving 70% or greater confluence. Additional doublings are achieved through the use of a state of the art Quantum bioreactor (Cerus, Concord, California). Final harvest yields vials for long term storage in vapor state LN2 tanks. Cell counts acquired by testing on Chemometic N202 cell counter (Gydevang, Denmark).
$749 per vial
Images Courtesy of Emma Dong at LSUHSC
Amnion
Storage
20C or in vapor state LN2 until ready to use
Sterility
Products are tested and found to be negative for mycoplasma and endotoxin.
Infectious Diseases
Donor tested and found to be negative for HIV 1, HIV 2, Hepatitis B, Hepatitis C, HTLV 1, WNV, Zika, and Syphilis
Methods
Human amnion is isolated from the placenta, washed in phosphate buffered saline and packed for long term storage in a 20C freezer